University of PardubiceFaculty of Chemical Technology

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Comparison of toxic effects of acetaminophen, glutathione and an acetaminophen-metabolite in liver mitochondria

Authors: Roušarová Erika | Vrbová Martina | Čapek Jan | Kučera Otto | Červinková Zuzana | Roušar Tomáš

Year: 2015

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Comparison of toxic effects of acetaminophen, glutathione and an acetaminophen-metabolite in liver mitochondria	Acetaminophen belongs among mostly used antipyretics and analgesics. It is considered to be safe at therapeutic doses. However, after overdose, it can lead to hepatotoxicity followed by acute liver failure. The cause of cell damage has been found in its activation to an oxidized compound that is detoxified through glutathione. However, some studies showed that glutathione conjugates may exert also their toxic effects. Therefore, the aim of our study was to describe the effect of acetaminophen (APAP), its metabolite and glutathione in isolated liver mitochondria. We used rat, murine and also human isolated mitochondria for determination of reactive species and for characterization of cellular respiration. We used a fluorescence probe DCFDA to determine production of reactive species and respirometry to characterize the cellular respiration in presence of tested compounds. We found that mitochondrial function was inhibited in presence of newly synthetized APAP-related metabolite.