Publication detail

Evaluation of use of HK-2 cells for nephrotoxicity testing in vitro.

Authors: Roušar Tomáš | Vrbová Martina | Roušarová Erika | Čapek Jan | Česla Petr

Year: 2015

Type of publication: ostatní - článek ve sborníku

Name of source: Applied Natural Sciences 2015: conference proceedings

Publisher name: Univerzita sv. Cyrila a Metoda v Trnave

Place: Trnava

Page from-to: 137

Titles:

Language	Name	Abstract	Keywords
eng	Evaluation of use of HK-2 cells for nephrotoxicity testing in vitro.	Estimation of nephrotoxicity in vitro is a common approach for testing of toxicity in kidney instead of in vivo conditions. There have been used numerous types of cells, e.g. isolated primary kidney cells and animal (LLC-PK1, MDCK) or human cell lines (HK-2). Recently, Human Kidney 2 cells (HK-2) have become the mostly used in vitro kidney model. On the other hand, some questions about their general use in toxicity research remain to be solved because a number of characteristic properties of HK-2 cells have not been described yet. Therefore, the goal of our study was to describe the possibility of HK-2 to produce specific renal toxicity markers and to respond to toxic stimuli. We cultured HK-2 cells for 24 hours and characterized their ability to produce NGAL and KIM-1 kidney toxicity markers. As well, we estimated the possibility of metabolic activation of a typical phase-I drug, acetaminophen. We found that HK-2 cells are able to produce both NGAL and KIM-1 markers in response to toxic compounds. As well, we found that HK-2 cells contain numerous mitochondria usable for detection of a mitochondrial impairment. Finally, our finding of oxidation products of acetaminophen proved that HK-2 cells also contain specific types of cytochromes.

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