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Proteomic investigation of biomarkers associated with regression of tumor diseases after HDT/ASCT

Authors: Kročová Eliška | Jankovičová Barbora | Kupčík Rudolf | Dvořáková Veronika | Lakota Ján | Bílková Zuzana

Year: 2015

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Proteomic investigation of biomarkers associated with regression of tumor diseases after HDT/ASCT	Introduction and Objectives: There is increasing evidence that autoimmunity can control the tumor in variety of malignancies [1]. In our case we focused on patient with multiple myeloma who developed an aplastic anemia type syndrome together with high titer of anti-carbonic anhydrase I autoantibodies after high dose therapy (HDT) with autologous stem cell transplantation (ASCT). Tumor regression without relapse was observed in this patient [2]. The mechanism of this phenomenon is still unclear, so we continue in screening the patients´ sera to identify other associated interactive protein(s). Methods: Firstly, we separated lysates of two different human cell lines (adherent breast adenocarcinoma cells - SKBR3 and suspension erythroleukemia cells - HEL92.1.7) using two-dimensional gel electrophoresis (2-D SDS-PAGE) followed with western-blot (WB) analysis for identification of proteins immunoreactive with applied sera. Detected protein spot(s) were excised from 2-D gel and after in-gel digestion were analyzed by MS and MS/MS (MALDI LTQ Orbitrap XL). Obtained results were confirmed by using commercial antigen(s). Results and Discussion: We observed by SDS-PAGE/WB analysis with chemiluminescence detection one abundant protein spot (Mw 48 kDa with basic character). Protein α-enolase was identified by MS analysis, which is known as tumor-associated antigen inducing specific immune responses in cancer patients [3]. Conclusions: We hope that this information could be helpful for explanation of the described phenomenon and applicable in various diagnostic and therapeutic approaches.	α-enolase, carbonic anhydrase I, tumor regression, autoimmunity, serological proteome analysis