University of PardubiceFaculty of Chemical Technology

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SIMULTANEOUS ANALYSIS OF SIGNIFICANT OVARIAN CANCER BIOMARKERS BY FLOW-CELL SCREEN PRINTED ELECTRODES

Authors: Čadková Michaela | Dvořáková Veronika | Kovářová Aneta | Metelka Radovan | Bílková Zuzana | Korecká Lucie

Year: 2016

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	SIMULTANEOUS ANALYSIS OF SIGNIFICANT OVARIAN CANCER BIOMARKERS BY FLOW-CELL SCREEN PRINTED ELECTRODES	Diagnosis of ovarian cancer is mostly based on single analysis of significant biomarkers CA125 and human epididymal protein-4 (HE4)1. On the other hand, precise and early determination of multiple tumor markers could greatly improve the treatment efficiency and all performed in one analysis can considerably save costs, sample volume and time. The key elements of simultaneous analysis are varyin signal probes of detection antibodies. Our developed immunosensor is based on advantageous combination of magnetic separation enhancing the reaction of specific antibodies and target antigen and secondary antibodies labeled by quantum dots (QDs). Due to their different chemical composition (usually Cd, Zn, Pb, In or Ga compounds)2 the resulting electrochemical response of each metal appears at characteristic potential without any overlaps. Therefore, quantum dots together with their electrochemical detection have great potential for multiparametric analysis of biomarkers of interest. The goal was to select suitable kinds of quatum dots for simutaneous detection in microfluidic screen printed electrodes. In addition, the potential of sensitive simultaneous detection of biomarkers is boosted using the microfluidic device, flow-cell screen printed electrodes respectively, which take advantage of circulation of released metal ions of quantum dots and thus their enhanced accumulation on working electrode surface resulting in more sensitive analysis compare to commonly used screen printed electrodes.	elctrochemical immunosensor; quantum dots; flow-cell screen printed electrodes;