Publication detail

Quantum dots-modified silica nanoparticles as immunosensing platform allowing simultaneous electrochemical detection of cancer biomarkers

Authors: Kovářová Aneta | Drbohlavová Jana | Bílková Zuzana | Korecká Lucie

Year: 2017

Type of publication: ostatní - článek ve sborníku

Name of source: YISAC 2017 24th Young Investigators Seminar on Analytical Chemistry : book of abstracts

Publisher name: Ca’ Foscari University of Venice

Place: Benátky

Page from-to: 3

Titles:

Language	Name	Abstract	Keywords
eng	Quantum dots-modified silica nanoparticles as immunosensing platform allowing simultaneous electrochemical detection of cancer biomarkers	Nowadays, there is an effort to determine simultaneously two or more biomarkers within one analysis allowing to improve selectivity in diagnosis of the disease. Quantum dots (QDs), semiconductor nanoparticles composed of various heavy metals, could be advantageously used as labels of antibodies in QDs-linked immunosorbent assays (QLISA). Electrochemical detection of QDs, based on monitoring of current response of metal ions released from QDs after dissolution in acetic medium, provide us the possibility of multiple biomarkers analysis. Moreover, mesoporous silica nanoparticles (SiNPs) are useful in bioanalyses for their large surface area. These features of SiNPs enable to bind higher amounts of QDs to SiNPS and therefore to antibodies leading to increased current response. Specific antibodies allowing detection of protein biomarkers of ovarian cancer, α-fetoprotein (AFP) and epididymal protein 4 (HE4), were prepared and used for construction of sensitive magneto-immunosensor. Square wave anodic stripping voltammetry (SWASV) was used as a detection technique and measurements were realised on bismuth film modified carbon screen printed electrodes (BiSPCE).	silica nanoparticles; quantum dots; SWASV; screen-printed electrodes; antibody labelling

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