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Neutrophil gelatinase-associated lipocalin production negatively correlates with HK-2 cell impairment: Evaluation of NGAL as a marker of toxicity in HK-2 cells
Authors: Hauschke Martina | Roušarová Erika | Flidr Pavel | Čapek Jan | Libra Antonín | Roušar Tomáš
Year: 2017
Type of publication: článek v odborném periodiku
Name of source: Toxicology in Vitro
Page from-to: 52-57
Titles:
Language Name Abstract Keywords
cze NGAL produkce negativně koreluje s poškozením HK-2 buněk. NGAL produkce negativně koreluje s poškozením HK-2 buněk. NGAL je extracelulární protein produkovaný převážně v ledvinách. Nedávno byl použit jako slibný biomarker pro detekci renálního poškození in vivo. Přesto dosud nebyl použit jako biomarker in vitro, a ověření tohoto bylo hlavním cílem naší studie. Viabilita buněk; HK-2 buňky; in vitro neforotoxicita; NGAL; markery nefrotoxicity
eng Neutrophil gelatinase-associated lipocalin production negatively correlates with HK-2 cell impairment: Evaluation of NGAL as a marker of toxicity in HK-2 cells Neutrophil gelatinase-associated lipocalin is an extracellular protein produced mostly in kidney. Recently, it has become a promising biomarker of renal damage in vivo. On the other hand, the validation of NGAL as a biomarker for nephrotoxicity estimation in vitro has not been characterized in detail yet. Since the HK-2 cells are frequently used human kidney cell line, we aimed to characterize the production of NGAL in these cells and to evaluate NGAL as a possible marker of cell impairment. We used heavy metals (mercury, cadmium), peroxide, drugs (acetaminophen, gentamicin) and cisplatin to mimic nephrotoxicity. HK-2 cells were incubated with selected compounds for 1-24h and cell viability was measured together with extracellular NGAL production. We proved that HK-2 cells possess a capacity to produce NGAL in amount of 2pg/ml/h. We found a change in cell viability after 24h incubation with all tested toxic compounds. The largest decrease of the viability was detected in mercury, acetaminophen, cisplatin and gentamicin. Unexpectedly, we found also a significant decrease in NGAL production in HK-2 cells treated with these toxins for 24h: to 11±5%, 54±5%, 57±6% and 76±9% respectively, compared with controls (=100%). Our results were followed with qPCR analysis when we found no significant increase in LCN2 gene expression after 24h incubation. We conclude that extracellular NGAL production negatively correlates with HK-2 cell impairment. Cell viability; HK-2 cells; In vitro nephrotoxicity; NGAL; Nephrotoxicity markers

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