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COMPARISON OF ACETAMINOPHEN AND SALAZOPYRIN HEPATOTOXICITY IN VITRO

Authors: Roušar Tomáš | Hauschke Martina | Brůčková Lenka | Čapek Jan | Česla Petr

Year: 2017

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	COMPARISON OF ACETAMINOPHEN AND SALAZOPYRIN HEPATOTOXICITY IN VITRO	Acetaminophen (acet-p-aminophenol) is one of the mostly used antipyretics and analgesics. It is an over-the-counter drug so that its overdose can often lead to toxicity occurring in liver and kidney especially. Therefore, acetaminophen (APAP) is a typical hepatotoxic compound causing even acute liver failure. Another structurally related compound, salazopyrin, has been used also frequently for a treatment in men. Salazopyrin (SAL) has been used mostly to treat inflammatory bowel diseases, i.e. rheumatoid arthritis, ulcerative colitis, and Crohn's disease. The adverse effects of SAL treatment can occur also in kidney and liver and that is why we aimed to compare the hepatotoxicity of both compounds in liver cells in vitro. The cells were treated with both tested compounds in the range of concentrations 0-10 mmol/l up to 24 hours. We estimated cell viability after the treatment using a spectrophotometric cell proliferation reagent. In addition, we assessed changes in MMP. We evaluated the toxic effect as a decrease in cell viability in comparison to controls. We found that both tested compounds were capable of cellular impairment. Especially, after 24h treatment, the toxic effects were found; e.g. APAP (5 mmol) induced a significant reduction of cell viability by 23±5% and SAL (1 mmol) decreased cell viability significantly by 24±1%. Similar results were found in MMP characterization, where a significant toxic effect was found in all tested concentrations of both compounds. We conclude that the extent of cell impairment induced by salazopyrin can be similar to that caused by acetaminophen.	acetaminophen; salazopyrin; hepatotoxicity in vitro