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Electrochemical immunosensor based on antibody-nanoparticle conjugates as a potential tool for rapid detection of foodborne pathogenes
Authors: Krulišová Pavla | Kovářová Aneta | Korecká Lucie | Bílková Zuzana
Year: 2017
Type of publication: ostatní - článek ve sborníku
Name of source: YISAC 2017 24th Young Investigators Seminar on Analytical Chemistry : book of abstracts
Publisher name: Ca’ Foscari University of Venice
Place: Benátky
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Electrochemical immunosensor based on antibody-nanoparticle conjugates as a potential tool for rapid detection of foodborne pathogenes Food borne illnesses caused by pathogens and their toxins pose serious threat for human health. There are many pathogens identified as cause of food-borne outbreaks but for the most of them are responsible especially Salmonella spp., Listeria monocytogenes and Escherichia coli. The conventional methods for their detection comprise of pathogens pre-enrichment, selective enrichment and selective plating followed by standard biochemical identifications, methods of molecular biology as well as serological confirmation. While these conventional methods are generally regarded as a “golden standard”, they are considerably limited by assay time which can be up to one week. Therefore, development of more efficient and faster method allowing direct detection and identification of food-borne pathogen bacteria is now of great importance worldwide. One of very promising tools seems to be electrochemical immunosensor generally based on reaction of detected bacteria and two specific antibodies. One serves, in combination with magnetic particles, for efficient capture of determined bacteria even from the complex sample. Second antibody is responsible for generation of final detection signal originated from sensitive probe linked to the antibody molecule. In this study, we have focused on the use of new types of probes, concretely gold nanoparticles and quantum dots. Square wave stripping voltammetry was used as detection method. Due to its simplicity and timelessness this type of biosensor, especially in combination with miniaturized disposable screen-printed electrodes, which are used for analysis, should be promising for use in quickly screening bacterial contamination in food . foodborne pathogens; magneto-immuno capture; electrochemical detection

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