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Evaluation of Method Based on Vacuum Assisted Solid Phase Microextraction for Analysis of Beer Volatile Profiles

Authors: Adam Martin | Adámková Karolína | Eisner Aleš | Bajer Tomáš | Ventura Karel

Year: 2017

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Evaluation of Method Based on Vacuum Assisted Solid Phase Microextraction for Analysis of Beer Volatile Profiles	Non-equilibrium SPME sampling under vacuum conditions may dramatically improve extraction kinetics compared to SPME under atmospheric pressure. Although reduced pressure conditions during SPME sampling are not expected to increase the amount of analytes extracted at equilibrium, reducing the total pressure will increase evaporation rates and result in a faster overall extraction process. The reduced pressure speeds partitioning of the semivolatile compounds into the gas phase and reduces the boundary layer around the SPME fiber, which enables a fraction of the volatilized organics to partition into the SPME fiber. The main aim of this study was the evaluation of a modern sampling strategy based on the use of Vac-SPME procedure and its applicability to the analysis of volatile profile of various beer samples. The separation and identification of target compounds was performed by GC–MS method together with comparison of linear retention indices of individual compounds with those found in spectral libraries and/or available in on-line databases. As an analytical tool, experimental designs were used for the method optimisation. First of all, the most significant factors affecting the whole procedure were evaluated by the Plackett– Burman design. After that, the final experimental conditions were established by five-level orthogonal central composite design followed by response surface modelling. The proposed method was compared to conventional method based on the conventional SPME approach under atmospheric pressure. It was confirmed that Vac-SPME approach provides a sufficient better results considering the response in terms of both the total peak areas and mainly the number of identified compounds, where more volatile components were detected together with less volatile substances. The use of Vac-SPME procedure proved to be a very good alternative for the screening of volatile compounds present in beer with a wider range of volatilities.	SPME; GC-MS; Beer Volatile Profile