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Electrochemical immunosensor for rapid and specific detection of foodborne pathogens
Authors: Murasová Pavla | Kovářová Aneta | Korecká Lucie | Bílková Zuzana
Year: 2017
Type of publication: ostatní - článek ve sborníku
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Electrochemical immunosensor for rapid and specific detection of foodborne pathogens Foodborne illnesses caused by pathogens and their toxins pose serious threat for human health. Many pathogens have been identified as a cause of food-borne outbreaks but for the most of them are responsible especially Salmonella spp., Listeria monocytogenes and Escherichia coli. Standard methods including several consecutive steps as culture enrichment, selective enrichment and plating followed by standard serological or biochemical confirmation are considered as a gold standard. Time-consuming selective plating steps could be replaced by qPCR or ELISA assays, but this strategy has led to shortening of detection time from the current 5 days to just 2 - 3 days, the positive samples must be still confirmed by traditional microbiological approach. Therefore, the development of rapid (in hours), sensitive/specific and robust method for foodborne pathogens detection continues to be a challenge. Our strategy suitable preferably for liquid samples combines immunomagnetic separation step with direct electrochemical detection of the captured bacteria cells by square wave stripping voltammetry sensor. Captured bacteria cells in a complex with magnetic beads are labelled by electrochemically active probes, specifically e.g. gold nanoparticles or quantum dots functionalized by specific IgG molecules. Square wave stripping voltammetry was used as a detection technique for analysis of metal ions released from the aforementioned probes by acid hydrolysis. Magnet located near the miniaturized disposable screen-printed electrodes attracts the labelled complexes, and released metal ions provide proper measurable signal. This analytical strategy leads to the results in a few hours Electrochemical immunosensor; foodborne pathogens; magneto-immuno capture

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