University of PardubiceFaculty of Chemical Technology

University

Employees
|
Students
Česky

Publication detail

Electrochemical magneto-immunosensors for analysis of single or multiple disease biomarkers

Authors: Korecká Lucie | Kovářová Aneta | Murasová Pavla | Kašparová Jitka | Sýs Milan | Metelka Radovan | Bílková Zuzana

Year: 2017

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Electrochemical magneto-immunosensors for analysis of single or multiple disease biomarkers	Analyses of protein biomarkers or allergens, associated with several more or less serious diseases, in clinical laboratories are most often based on common immunoanalytical methods. The main principle of sandwich type immunoassay consists in specific reaction of analysed biomarker and two antibodies. One serves for protein capture and second, labelled by eligible tag, as a signal generating antibody. Final sensitivity of the assay is given by the antibody label which could be enzyme, fluorochrome, chemiluminescent substance or nanoparticle. Moreover combination with easily separable magnetic microparticles used as solid phase for fixation of capture antibody enable analysis of complex biological samples without lengthy pre-treatment. Electrochemical immunosensors abound numerous benefits, such as selectivity, simple instrumentation, broad spectrum of detection techniques etc. Also the possibility of the use of screen-printed sensors, characterized by low-cost fabrication, low sample consumption and possible surface modification is beneficial. Recently, increasing number of application of quantum dots (QDs) as nanolabels of antibodies in combination with electrochemical immunosensors is mentioned. These surface-functionalized semiconductor nanocrystals made of various materials (Zn, Cd, In, Ga) could be simply analysed by square wave anodic stripping voltammetry on thin-film modified screen-printed electrodes (mercury or bismuth film). Here, we present the overview in concepts of our electrochemical magneto-immunosensors for sensitive analysis of selected ovarian cancer biomarkers (proteins HE4, CA125, AFP). In its development several labels of antibody, from common enzymes, through nanoparticles to quantum dots from the perspective of single or multiple biomarker assay were utilized.	Immunoassays;Biosensors;Electrochemical detection;Screen-printed electrodes