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Why is the hydrolytic activity of acetylcholinesterase pH dependent? Kinetic study of acetylcholine and acetylthiocholine hydrolysis catalyzed by acetylcholinesterase from electric eel
Authors: Komersová Alena | Kovářová Markéta | Komers Karel | Lochař Václav | Čegan Alexander
Year: 2018
Type of publication: článek v odborném periodiku
Name of source: Zeitschrift für Naturforschung C = A Journal of Biosciences
Publisher name: Walter de Gruyter GmbH & Co. KG
Place: Berlin
Page from-to: 345-351
Titles:
Language Name Abstract Keywords
cze Proč je hydrolytická aktivita acetylcholinesterázy závislá na pH? Kinetická studie hydrolýzy acetylcholinu a acetylthiocholinu katalyzované acetylcholinesterázou z elektrického úhoře Závislost hydrolytické aktivity acetylcholin esterázy na pH byla studována v rozsahu pH 4,8-9,8 (fosfátový pufr) při teplotě 25°C a iontové síle 0,11 M. Počáteční koncentrace substrátu byla 4 mM. Závislost aktivity enzymu na pH pro oba studované substráty ve výše uvedeném rozmezí pH poskytuje sigmoidální křivku s maximální katalytickou aktivitou při pH 8-9,5. acetylcholiesteráza; enzymatická aktivita; kinetika; závislost na pH
eng Why is the hydrolytic activity of acetylcholinesterase pH dependent? Kinetic study of acetylcholine and acetylthiocholine hydrolysis catalyzed by acetylcholinesterase from electric eel The dependence of the activity of acetylcholinesterase from electric eel at a pH value range of 4.8-9.8 (phosphate buffer), regarding acetylcholine and acetylthiocholine hydrolysis, was determined at 25 degrees C, ionic strength of 0.11 M, and initial substrate concentration of 4 mM. At a pH range of 4.8-9.8, the dependences A(pH) form a sigmoid increasing curve with the maximum catalytic activity at a pH range 8 9.5. For acetylcholine hydrolysis, the kinetic reason for such an increase in A consists mainly of an increase in the rate constant k(2) (Michaelis-Menten) model with increasing pH of the reaction mixture. For acetylthiocholine hydrolysis, the kinetic explication of the determined dependence A(pH) is more complicated. acetylcholinesterase; enzyme activity; kinetics; pH dependence