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ELECTROCHEMICAL IMMUNOSENSOR FOR RAPID DETECTION OF WHOLE BACTERIAL CELLS USING METAL NANOPARTICLES AS ANTIBODY LABEL
Authors: Korecká Lucie | Murasová Pavla | Kovářová Aneta | Kašparová Jitka | Bílková Zuzana
Year: 2018
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng ELECTROCHEMICAL IMMUNOSENSOR FOR RAPID DETECTION OF WHOLE BACTERIAL CELLS USING METAL NANOPARTICLES AS ANTIBODY LABEL There are many pathogens, or their toxins cause of food-borne illnesses but especially Salmonella spp., Listeria monocytogenes and Escherichia coli are the most risky and pose serious threat for human health [1,2]. The conventional methods for their detection comprise of pathogens pre-enrichment, selective enrichment and selective plating with subsequent biochemical identification, or confirmation through serological or molecular biology methods. While these conventional methods are generally regarded as a “golden standard”, they are considerably limited by long assay time (up to one week) [3]. Therefore, there is a great effort to develop rapid and more efficient method allowing direct detection and identification of bacterial cells. One of very promising tools seems to be electrochemical immunosensors generally based on recognition of detected bacteria by two specific antibodies. One serves, in combination with magnetic particles, for efficient capture of determined bacteria even from the complex sample. Second antibody is responsible for finally monitored electrochemical signal originated from label linked to the antibody molecule. Here, we present the use of gold nanoparticles and quantum dots-based labels of antibodies enabling the electrochemical detection. Square wave stripping voltammetry was used as detection method combined with disposable screen-printed carbon electrodes. Presented arrangement of magneto-immunocapture combined with electrochemical detection allows shorten the analysis time from several days to four hours.

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