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TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles: A Tool to Selectively Isolate Biomolecules
Authors: Kupčík Rudolf | Macák Jan | Řehulka Pavel | Sopha Hanna Ingrid | Bílková Zuzana
Year: 2018
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles: A Tool to Selectively Isolate Biomolecules Isolation, study of a function and in vitro synthesis of biomolecules is of essential importance in many objects of life sciences. For structural analysis the efficient enrichment and purification are crucial for both, natural and recombinant biomolecules. Today there are a number of commercially available carriers that allow us to isolate and purify biomolecules of different nature, but we still face certain shortcomings, limitations which complicate separation process and increase the cost of final product. For example, widely employed technique Immobilized-Metal Affinity Chromatography (IMAC) exploits the metal chelate affinity of His-tagged recombinant molecules to obtain bioactive molecules in proper purity for next applications. However, this approach has several drawbacks, such as insufficient purity of recombinant proteins for in vivo applications and toxicity of released ions used for charging of chelating groups on polymeric resin surface. Also study of protein phosphorylation as one of the most important posttranslational modifications, which alters the biomolecules conformation and modify their function, needs suitable tool for effective enrichment of (multi)phosphoproteins/peptides. And again, using the traditional carriers as IMAC with captured metal ions (e.g. Fe3+) or metal oxides (e.g. TiO2) were faced some drawbacks. TiO2 based materials bind phosphopeptides in general, but elution of multiphosphorylated peptides is difficult due to their high affinity to TiO2. The aim of our work is to demonstrate the unique characteristics of a new nanotubular TiO2-based material decorated by Fe3O4 nanoparticles. Compared to common commercially available materials we would like to document high selectivity of this carrier for both types of molecules (His-tagged proteins and phosphopeptides), two tailored protocols included. Phosphopeptides: The phosphopeptides enrichment was performed either with TiO2 microspheres (Titansphere, GL Sciences) or with the novel magn His-tagged proteins; phosphopeptides; TiO2; Fe3O4

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