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TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles: A Tool to Isolate Biomolecules Containing Phosphate Backbone

Authors: Kupčík Rudolf | Macák Jan | Smělá Denisa | Řehulka Pavel | Řehulková Helena | Kročová Eliška | Sopha Hanna Ingrid | Fabrik Ivo | Bílková Zuzana

Year: 2018

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles: A Tool to Isolate Biomolecules Containing Phosphate Backbone	TiO2 is widely used as a material interacting with biomolecules due to its biocompatibility, stability or selectivity (R. Kupcik et al., ACS Applied Materials & Interfaces, 2017). Electrostatic interactions with phosphate groups in phosphopeptides with TiO2 has been widely employed prior to their enrichment (K. Engholm-Keller et al., Proteomics, 2013). Other important biomolecules containing phosphate moieties interacting with TiO2 are nucleic acids. For isolation of biomolecules, an affinity chromatography is widely preferred due to high clearance of impurities, however current materials and techniques have several drawbacks, such as insufficient selectivity. The aim of our work is to demonstrate the unique characteristics of TiO2 nanotubes decorated by Fe3O4 nanoparticles (TiO2NTs@Fe3O4NPs) that provide both higher selectivity for phosphopetides (as compared to commercial particles) and capability to isolate nucleic acids. For the both applications, cell lysates were used as an initial sample. Phosphopeptides were enriched after tryptic digestion of proteins in the lysate and analyzed using MALDI-MS and nLC-ESI-MS/MS. Nucleic acids were pre-isolated from the lysate, spiked with defined oligonucleotides and analyzed with gel electrophoresis before and after TiO2NTs@Fe3O4NPs isolation. As compared to commonly used TiO2 microspheres, TiO2NTs@Fe3O4NPs showed an enhanced selectivity for phosphopeptides and presence of non-phosphorylated peptides in the eluate was significantly reduced. It was also possible to isolate nucleic acids which means not only their interaction but also their elution. Finally, the material shows magnetic properties applicable for simple and quantitative separation from complex liquid samples.	TiO2 nanotubes; phosphopeptides; nucleic acids