Login for students

Login for employees

Publication detail

Development of the magnetic carrier for the anti-beta amyloid 1-42 magnetic ELISA assay
Authors: Slováková Marcela | Murasová Pavla | Vozandychová Věra | Bílková Zuzana
Year: 2019
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Development of the magnetic carrier for the anti-beta amyloid 1-42 magnetic ELISA assay Objectives: An occurrence of the natural specific antibodies against the beta-Amyloid 1-42 in patient sera with neurodegenerative disorders is examined using the magnetic ELISA with a newly developed magnetic solid phase. Magnetic solid phase preparation comprised the beta-Amyloid 1-42 immobilization on superparamagnetic magnetic beads. A major effort was made to reduce the nonspecific sorption of serum contaminants to the prepared bioaffinity magnetic carriers. Further, experiments testing the in vitro cytotoxicity of selected magnetic beads show their safe disposal. Methods: Superparamagnetic beads ProMag carboxyl and Micromer Amine varying in composition and size were characterized by scanning electron microscopy, dynamic light scattering and zeta potential measurement. Activation of superparamagnetic beads was performed by carbodiimide chemistry followed by beta-Amyloid 1-42 peptide immobilization. Unoccupied functional groups of beads were blocked by various low molecular polyethylene glycol agents or BSA. In vitro cytotoxicity test (XTT) was applied to assay the cytotoxicity of the selected superparamagnetic beads. Results: The bioaffinity magnetic carriers using ProMag carboxyl and Micromer Amine carrying the beta-Amyloid 1-42 ligand were successfully prepared. Techniques affecting the performance the non-specific sorption of the beads were evaluated. Both magnetic carriers were applied for magnetic ELISA detection of anti-beta-Amyloid 1-42 natural antibodies in human healthy and patient sera with neurodegenerative disorders. Conclusions: Analysis of patient sera with neurodegenerative disorders confirmed applicability of the newly developed anti-beta-Amyloid 1-42 bioaffinity magnetic carriers in the magnetic ELISA assay. Selected superparamagnetic particles can be considered as non-toxic. carrier; anti-beta amyloid 1-42; magnetic; ELISA;

Departments

Other Parts

Map

en.mapy.cz

Services

Popular Links

© 2023 University of Pardubice, Studentská 95, 532 10 Pardubice 2
Phone: +420 466 036 111, 466 036 112, 466 036 113
Webmaster, RSS
Data box ID: f5vj9hu
Accessibility Statement
CC BY-NC-ND 4.0Creative CommonsAttributionNonCommercialNoDerivatives