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Publication detail

The cytotoxicity of TiO2-based nanomaterials in macrophages
Authors: Smělá Denisa | Macák Jan | Taniguchi Akiyoshi | Bílková Zuzana
Year: 2021
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng The cytotoxicity of TiO2-based nanomaterials in macrophages Titanium dioxide (TiO2) in nanoform is a largely utilized material worldwide. The health hazard associated with this type of material attracted attention towards the research regarding its safety. The first interaction after the exposure of the human body to TiO2 nanomaterials (NMs) is with immunocompetent cells. The human myelogenous leukemia cell line THP-1 was used as a model system for our experiments, after their differentiation into macrophage-like cells using phorbol-12-myristate-13-acetate. The cell response varied based on the NMs characteristics, including their concentration, size, length, shape, chemical composition, or crystalline form. In this work, we tested TiO2-based NMs to study their cytotoxicity after interaction with macrophage-like cells and their ability to induce reactive oxygen species (ROS) production. The NMs were: TiO2 nanofibers (rutile crystalline form), two types of TiO2-based nanotubes (amorphous crystalline form with the inner diameter of 230 nm, length 5 µm either naked or coated by Fe3O4), and two types of commercial nanoparticles (anatase crystalline form, size <25 nm, and rutile crystalline form, size <100 nm). The shape and crystalline form of the NMs are important factors in cellular response – rutile crystalline form, in general, lowered the cell viability compared to the anatase or amorphous form. Rutile nanofibers caused a significant increase in ROS production, but rutile nanoparticles did not. The properties of the tested NMs are crucial for cell and tissue toxicity/biocompatibility, and it is important to carefully and step-by-step evaluate all forms of TiO2-based NMs. This approach enables us to utilize their benefits safely. titanium dioxide; nanomaterials; cytotoxicity; nanofibers; nanotubes; nanoparticles

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