University of PardubiceFaculty of Chemical Technology

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The preparation of hyaluronic acid nanoparticles as potential drug delivery carrier

Authors: Mannová Nikola | Korecká Lucie | Bílková Zuzana

Year: 2021

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	The preparation of hyaluronic acid nanoparticles as potential drug delivery carrier	Hyaluronic acid (HA) is a naturally occurring long linear polysaccharide known for its biocompatibility, biodegradability and non-immunogenicity. It has an important ability to interact with a specific receptor (CD44) on the surface of cancer cells. Thus HA has a great potential as carrier for drug delivery systems. In this study, we prepared hyaluronic acid nanoparticles (HA-NPs) by cross-linking of the linear polymer HA molecule. Specifically, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and adipic acid dihydrazide (ADH) were used as cross-linking agents in acetone medium. The product (HA-NPs) was then measured by a dynamic light scattering (DLS). Thanks to the broad size distribution of nanoparticles prepared by this method, it was necessary to find out which condition of the preparation corresponds with the final size distribution. For these reasons, the following conditions were altered and studied – the incubation time and the number of steps in the final acetone addition, the amount of ADH and the speed of mixing during incubation. The type of motion which seems to be the most convenient for this preparation is the medium speed rotation. The additions of ADH and acetone are the variables in altering the final size of nanoparticles. Two steps addition of acetone with incubation time 10 minutes corresponds with the size around 120 nm. For nanoparticles with the size around 100 nm, the best is the two steps addition of acetone with incubation 20 minutes. And for nanoparticles with size around 80 nm use the addition of acetone three times with incubation 30 minutes on rotator.	drug delivery carrier; drug delivery; hyaluronic acid; nanoparticles; preparation; optimization;