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TiO2 magnetic nanotubes for RNA isolation from complex biological samples

Authors: Smělá Denisa | Macák Jan | Bílková Zuzana

Year: 2022

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	TiO2 magnetic nanotubes for RNA isolation from complex biological samples	Commonly used methods for RNA isolation from biological samples are based on liquid-liquid extraction or solid-phase extraction. Very often, the commercial kits and methods for RNA isolation use chemicals such as phenol and chloroform, which are hazardous to work with and can also cause contamination of the final product. We wanted to develop a new method of RNA isolation from cells or from biological fluids that would be safer to perform and possibly more affordable as well. It was previously described, that materials based on TiO2 have an affinity to nucleic acids thanks to their strong interactions with phosphate backbone. Nucleic acids are negatively charged and the charge of TiO2 under acidic conditions is strongly positive. In addition, it was suggested that the adsorption of DNA on TiO2 is caused by interaction between DNA and hydroxyl groups on the surface of TiO2. Similar principles apply for TiO2 interactions with RNA. In our work, we tested different materials based on TiO2 to determine their applicability for RNA isolation. Among other materials, we used newly developed TiO2 nanotubes coated with Fe3O4 (TiO2NTs@Fe3O4NPs, CEMNAT). Magnetic properties of this material offered advantage in higher affinity towards RNA as well as an easier and faster performance of the protocol. This material was also successfully used for SARS-CoV-2 viral RNA isolation.	TiO2 nanotubes; RNA isolation