University of Pardubice Faculty of Chemical Technology

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Evaluation of the biological effects of TiO2 NPs in human neuroblastoma cells in vitro

Authors: Jelínková Štěpánka | Báčová Jana | Čapek Jan | Roušar Tomáš

Year: 2023

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Evaluation of the biological effects of TiO2 NPs in human neuroblastoma cells in vitro	The increasing occurrence of titanium dioxide nanoparticles (TiO2 NPs) in the environment leads to increased exposure to human body, raising questions about their safety. After exposure, TiO2 NPs can enter the human body through the skin, gastrointestinal or respiratory tract. Then, TiO2 NPs can reach various organs, such as the liver, lungs, or brain. Therefore, testing the biological effects of TiO2 NPs in human neuronal cells is crucial to understanding the possible neurotoxicity of TiO2 NPs. Our aim was to evaluate the biological effect of commercially available TiO2 P25 NPs in human neuroblastoma cells (SH-SY5Y), which is a cell line commonly used in neurotoxicity testing. SH-SY5Y cells were exposed to 0-100 µg·mL-1 TiO2 P25 NPs for 24 and 48 h. Multi walled carbon nanotubes were used as a positive control. After incubation with nanomaterials, dehydrogenase activity and glutathione levels in SH-SY5Y cells were assessed. In addition, cell morphology was evaluated using fluorescence microscopy. The results showed a dose-dependent decrease of dehydrogenase activity in SH-SY5Y cells incubated with tested nanomaterials after 24 and 48 h. We detected changes in glutathione levels in SH-SY5Y cells after 24 and 48 h of incubation with TiO2 P25 NPs. Finally, our results were supported by fluorescence microscopy. We found changes in cell morphology after 24 and 48 h of treatment with 100 µg·mL-1 TiO2 P25 NPs. We concluded that tested TiO2 P25 NPs caused lower toxicity in SH-SY5Y cells compared to multi-walled carbon nanotubes.	Human neuroblastoma cells; TiO2 nanoparticles; in vitro