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Assessment of potential neurotoxicity of TiO2 nanoparticles

Authors: Jelínková Štěpánka | Báčová Jana | Čapek Jan | Roušar Tomáš

Year: 2023

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Assessment of potential neurotoxicity of TiO2 nanoparticles	The increased occurrence of titanium dioxide nanoparticles (TiO2 NPs) in the environment can lead to increased exposure in human body. Therefore, questions arise about the safety of TiO2 NPs. After exposure, TiO2 NPs can enter the human body through different routes and reach various organs such as the liver, lungs, or brain [1]. Thus, evaluating the biological effects of TiO2 NPs in human neuronal cells is crucial to understand their potential toxicity. The aim of our study was to evaluate the biological effects of commercially available TiO2 P25 NPs in human neuroblastoma cells (SH-SY5Y). The SH-SY5Y cell line is a commonly used cell model for neurotoxicity testing. SH-SY5Y cells were exposed to 0 100 µg·mL-1 TiO2 P25 NPs for 24 and 48 h. We used multi walled carbon nanotubes as a positive control. After incubation with nanomaterials, dehydrogenase activity and glutathione levels [2] in SH-SY5Y cells were evaluated. In addition, cell morphology was assessed using fluorescence microscopy. The results showed a dose-dependent decrease of dehydrogenase activity of SH-SY5Y cells incubated with tested nanomaterials for 24 and 48 h. We detected changes of glutathione levels in cells after 24 and 48 h of treatment with TiO2 P25 NPs. We found changes in cell morphology after 24 and 48 h of treatment with 100 µg·mL-1 TiO2 P25 NPs. We conclude that herein tested TiO2 P25 NPs caused lower toxicity in SH-SY5Y cells compared to multi walled carbon nanotubes.	Neurotoxicity; TiO2 nanoparticles; in vitro