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Evaluating neurotoxicity of TiO2 nanoparticles in vitro

Authors: Jelínková Štěpánka | Báčová Jana | Čapek Jan | Roušar Tomáš

Year: 2023

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Evaluating neurotoxicity of TiO2 nanoparticles in vitro	Due to the increased development, production, and occurrence of TiO2 nanoparticles (NPs) in the environment, the exposure in the human body is inevitable. Therefore, concerns arise about the safety of TiO2 NPs. The lung, gastrointestinal tract and skin are the most common entrance points of TiO2 NPs into the human body. TiO2 NPs pass through the vessel wall and circulate in the blood, from where they can be transported to organs. Because of their small size, TiO2 NPs can cross the blood-brain barrier and reach the brain [1]. TiO2 NPs can cause cytotoxicity and genotoxicity in neural cells. Thus, evaluating the biological effects of TiO2 NPs in neuronal cells is crucial to understand their potential toxicity. We aimed to evaluate the biological effects of commercially available TiO2 P25 NPs in human neuroblastoma cells (SH-SY5Y), which is a commonly used cell model for neurotoxicity testing. SH-SY5Y cells were treated with 0 100 µg·mL-1 TiO2 P25 NPs for 24 and 48 h. The multi walled carbon nanotubes inducing a large cell impairment were used as a positive control. After incubation with nanomaterials, dehydrogenase activity, glutathione levels [2] and nuclear condensation and fragmentation [3] in SH-SY5Y cells were evaluated. In addition, cell morphology was assessed using fluorescence microscopy. The results showed a dose-dependent decrease of the dehydrogenase activity and glutathione levels of SH-SY5Y cells incubated with tested nanomaterials for 24 and 48 h. We found that TiO2 P25 NPs induced the nuclear condensation and fragmentation in SH-SY5Y cells. We conclude that tested TiO2 P25 NPs caused significant level of toxicity in SH-SY5Y cells, but lower compared to multi walled carbon nanotubes.	Evaluating; neurotoxicity; TiO2; nanoparticles; in vitro