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Comparison of TiO2 Nanoparticle-Mediated Biological Effects in Human Cell Lines

Authors: Jelínková Štěpánka | Báčová Jana | Čapek Jan | Roušar Tomáš

Year: 2024

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Comparison of TiO2 Nanoparticle-Mediated Biological Effects in Human Cell Lines	The exponential growth of production and occurrence of TiO2 nanoparticles (NPs) in the environment leads to increased exposure to the human body raising questions about their safety. After exposure, TiO2 NPs can enter the human body through the respiratory tract, gastrointestinal tract, or the skin. Then, TiO2 NPs circulate in bloodstream and can be transported to various organs, such as the liver, kidney, or brain. Therefore, the assessment of potential cytotoxicity of TiO2 NPs in different human cells is crucial. There are only several studies comparing TiO2 NP induced toxicity using various human cell lines. Therefore, we aimed to compare the biological effects of commercially available TiO2 P25 NPs in human cell lines of different origin. We chose four human cell lines representing tissues possibly targeted by TiO2 NPs, i.e. pulmonary A549, neuronal SH-SY5Y, bone MG 63 and blood Jurkat cells. The cells were treated with TiO2 P25 NPs (0-100 µg·mL-1) for 24 and 48 h. The multi-walled carbon nanotubes inducing strong cellular damage were used as a positive control. After incubation with nanomaterials, cell viability and glutathione levels in cells were estimated to characterize biological effects. In addition, fluorescence microscopy was used to assess cell morphology. We found that the toxicity of TiO2 P25 NPs depends on the type of cell line and the exposure time. In conclusion, TiO2 P25 NPs caused mild cellular impairment in the case of SH-SY5Y and Jurkat cells compared to multi-walled carbon nanotubes.	TiO2 nanoparticles; cytotoxicity; human cell lines