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Toxicity assessment induced by TiO2 P25 nanoparticles in human neuroblastoma cells

Authors: Jelínková Štěpánka | Báčová Jana | Čapek Jan | Roušar Tomáš

Year: 2024

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Toxicity assessment induced by TiO2 P25 nanoparticles in human neuroblastoma cells	Over past years, the development, production and occurrence of TiO2 NPs have been steadily increasing, resulting in increased exposure to the human body. Therefore, concerns arise about the safety of TiO2 NPs. After entering the human through different entry points and subsequent blood transport, TiO2 NPs can be transported to organs. Due to their small size, TiO2 NPs can cross the blood-brain barrier and reach the brain. TiO2 NPs can cause cytotoxicity and genotoxicity in neural cells. Thus, it is crucial to evaluate the biological effects of TiO2 NPs in neuronal cells to understand their potential toxicity. We aimed to assess the biological effects of commercially available TiO2 P25 NPs in human neuroblastoma cells (SH-SY5Y). SH-SY5Y cells were treated with 0 100 µg·mL-1 TiO2 P25 NPs for 24-72 h and multi-walled carbon nanotubes were used as a positive control. After incubation with nanomaterials, dehydrogenase activity, glutathione levels, nuclear condensation and fragmentation and the superoxide production in SH-SY5Y cells were evaluated. The results showed a dose-dependent decrease of the dehydrogenase activity, changes in glutathione levels, the production of superoxide and nuclear condensation and fragmentation of SH-SY5Y cells incubated with tested nanomaterials for 24-72 h. In conclusion, tested TiO2 P25 NPs caused a significant level of toxicity in SH-SY5Y cells.	Toxicity assessment; TiO2 nanoparticles, human neuroblastoma cells