University of PardubiceFaculty of Chemical Technology

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Patient-derived gastric cancer model as a platform for in vitro cytotoxicity testing

Authors: Vlček Antonín | Pohnán Radek | Šmíd Lenka | Čapek Jan | Suchánek Štěpán | Bureš Jan | Kouhoutová Darina | Roušar Tomáš

Year: 2025

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

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Language	Name	Abstract	Keywords
eng	Patient-derived gastric cancer model as a platform for in vitro cytotoxicity testing	Gastric cancer remains one of the leading cancer types in terms of incidence and mortality in many countries around the world. Introducing new gastric cancer cell lines contributes to understanding gastric cancer's vast heterogeneity and helps us to establish personalized disease models. The continued significance of 2D models is attributed to their affordability, excellent reproducibility, and more straightforward interpretation compared to advanced culture systems. In this study, we employed a newly established patient-derived gastric cancer cell line (CGC) and compared it to the commercial cell line N87 to investigate the potential cytotoxicity of chemotherapeutics. We exposed gastric cancer cells, cultivated in 2D monolayers, to selected anti-cancer agents for 24h. These compounds included camptothecin (CPT; 1 and 5 µM), staurosporine (STA; 0,1 and 1 µM), cisplatin (CisPt; 25 and 75 µM), and 5-fluorouracil (100 and 2500 µM). To characterize a cytotoxic effect, we measured intracellular glutathione concentration, dehydrogenase activity, and ATP content as cellular damage indicators. We also assessed the morphological changes in treated gastric cancer cells by visualizing phalloidin-labeled actin filaments with fluorescence microscopy. Our results showed that all compounds tested induced a significant decrease in intracellular glutathione concentration, dehydrogenase activity, and ATP content. The cytotoxic effect was potentiated in a dose-dependent manner. We detected the most significant cytotoxic effect after the staurosporine and cisplatin treatment. The CGC cell line was much more sensitive to the induction of cellular damage compared to the N87 cell line, especially in the case of staurosporine and cisplatin. Morphological examination revealed a potent cytotoxic effect of the tested compounds that resulted in cytoskeletal system deterioration. The findings indicate that the primary gastric cancer cell line CGC can serve as a personalized disease model	gastric carcinoma; patient-derived cell line; cytotoxicity; glutathione; dehydrogenase activity