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Western Blot assay for Confirmation of Changes in CD44 Receptor Expression in Irradiated Lung-Derived Cells Treated with Hyaluronic Acid Nanoparticles
Authors: Kynclová Karolína | Carrillo Anna | Čížková Jana | Filipová Alžběta | Bílková Zuzana | Šinkorová Zuzana | Korecká Lucie
Year: 2025
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Western Blot assay for Confirmation of Changes in CD44 Receptor Expression in Irradiated Lung-Derived Cells Treated with Hyaluronic Acid Nanoparticles Hyaluronic acid (HA) is a biopolymer naturally occurring in extracellular matrix and synovial fluids, widely studied for its biocompatibility. To date, only a few studies have investigated the potential of HA as a radioprotective agent. It has been demonstrated that HA exhibits radioprotective effect in the intestinal epithelium through a Toll-like receptor 4 (TLR4) and cyclooxygenase-2 (COX-2)-mediated mechanism. However, HA’s use as a radioprotective substance is limited due to its radiation-induced fragmentation into short, inflammatory units. Nevertheless, HA in the form of nanoparticles exhibits improved stability and it has been proven that HA nanoparticles (HANPs), which were synthesized by optimized cross-linking of high-molecular weight HA are stable even upon irradiation and attenuate radiation-induced lung injury, particularly by modulating signaling pathways linked to fibrosis. HA’s key cell receptor is CD44, a ubiquitously expressed adhesion transmembrane glycoprotein that faciliates various biological activities, such as cell proliferation. CD44 is overexpressed in various types of cancer and is associated with poor prognosis and reduced response to radiation therapy. This is why, in this work, we aimed to confirm the changes in CD44 expression in lung-derived cells after irradiation, with or without pre-treatment with HANPs. For the confirmation of reactivity of HANPs with CD44 we used Western Blot analysis with chemiluminescent detection. We optimized the method and the interaction was confirmed. Our results also show the potential of HANPs as carriers for radioprotective agents and anti-cancer drugs. Western blot assay; CD44 Receptor; hyaluronic acid; nanoparticles

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