University of Pardubice Faculty of Chemical Technology

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Chip-based magnetic bioaffinity and enzyme reactors, development and applications

Authors: Slováková Marcela | Korecká Lucie | Jankovičová Barbora | Minc Nicolas | Bílková Zuzana

Year: 2007

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
cze	Magnetický bioafinitní a enzymové reaktory v čipovém uspořádání, vývoj a aplikace.	Anhydrotrypsin, porteináza K a specifické protilátky prot potravinovým alergenům byly imobilizovány na nově vyvinuté magnetické nosiče. Tyto IMER a IMAR byly použity v čipovém uspořádání ve spojení s MS detekcí.	mikročip, IMER, IMAR, anhydrotrypsin, proteináza K, ABeta peptidy, Tau proteiny
eng	Chip-based magnetic bioaffinity and enzyme reactors, development and applications	The technique of bioaffinity chromatography has potential uses in industry (food and drug processing), medicine (from routine clinical services to advances in medical technology) and in different research areas, i.e. simple enzyme purification, studies of enzyme mechanism, the isolation of specific receptor sites in cells and isolation of various biochemical markers. There is a continuous push to miniaturize and integrate as many laboratory instrumentation pieces as possible in the demands for a ?lab-on-chip?. Parallel to the boom of microfluidic systems, nanomaterials with paramagnetic properties have become a hot topic in recent research. Using carriers with magnetic property overcomes many of the problems associated with the use of liquid gel slurries. Magnetic particles provide universal system with additional convenience, consistency, stability, ease of handling and exceptional flexibility compared to standard chromatography resins. We focused on early diagnostics of neurodegenerative diseases (Alzheimer, prion disease) and validation of their possible markers. Especially the immunospecific supports dedicated to capturing and pre-concentration of target A peptides, Tau proteins or ?scrapie? prion molecule. Newly developed affinity carrier, based on immobilization of anhydrotrypsin onto magnetic beads and focused in magnetically active microfluidic device, was used for rapid isolation and pre-concentration of peptides containing specific amino acids on C - terminus of their molecules. This procedure benefits the simplification of mass spectrometry identification even in case of complex biological fluids. We use enzyme (IMERs) and immunoaffinity (IMARs) magnetic microreactors for epitope mapping of model food allergen ovalbumin. Magnetic enzyme microreactors were prepared for proteolysis of ovalbumin and subsequently the magnetic immunoaffinity microreactors with immobilized polyclonal and/or monoclonal anti-ovalbumin antibodies were utilized for ca	microchip; IMER; IMAR; anhydrotrypsin; proteinase K; ABeta peptides Tau proteins