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Application of Electrochemical Sensors for Determination of Anticholinesterase Activity and Immobilization of Enzyme
Authors: Vorčáková Katarína | Štěpánková Šárka | Zorič Petra | Sedlák Miloš | Metelka Radovan | Vytřas Karel
Year: 2012
Type of publication: článek ve sborníku
Name of source: Sensing in Electroanalysis Vol. 7
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 409-422
Titles:
Language Name Abstract Keywords
cze Využití elektrochemických sensorů pro stanovení anticholinesterázové aktivity a imobilizaci enzymu Cholinesterasy jsou významné enzmy zodpovědné za rychlou eliminaci acetylcholinu uvolňovaného na cholinergní synpsi. Aktivita cholinesteras je zvýšená u pacientů s Alzheimerovou chorobou, proto je důležité hledat nové inhibitory cholinesteras. Pro stanovení inhibiční účinnosti může být použito několik metod. V našem případě byla anticholinesterasova aktivity vyjádřená jako IC50 testována pomocí tištěných elektrod. Byly také zavedeny dvě metody imobilizace enzymu na povrch sensoru. Kinetické parametry (Km, Vm) byly stanoveny pro imobilizovaný a neimobilizovaný enzym. tištěné elektrody; square-wave voltametrie;cholinesterasy;IC50
eng Application of Electrochemical Sensors for Determination of Anticholinesterase Activity and Immobilization of Enzyme Cholinesterases are important enzymes, which are responsible for the rapid elimination of acetyclholine after its releasing at cholinergic synapses. High activity of cholinesterases is present in patients with Alzheimer´s disease. Therefore, testing of new inhibitors of cholinesterases are important. For determination of the inhibition efficiency several methods can be used. In our case, the anticholinesterase activity expressed by IC50 of five carbamate compounds were tested using screen-printed electrodes and square-wave voltammetry. The next part of our work was focused on testing of two types of immobilization of enzyme on the surface of screen-printed electrodes. Kinetic parameters (Km, Vm) were determined for immobilized and soluted enzyme. Obtained results were disscused. screen-printed electrodes; square-wave voltammetry; cholinesterases;IC50

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