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Electrochemical Sensors for the Estimation of the Inhibitory Effect of Phenylcarbamates to Cholinesterase
Year: 2015
Type of publication: článek v odborném periodiku
Name of source: Chemosensors
Place: Basel
Page from-to: 274-283
Titles:
Language Name Abstract Keywords
cze Elektrochemické senzory pro stanovení inhibičního účinku fenylkarbamatů na cholinesterázy U devíti látek ze skupiny fenylkarbamátů byla sledovaná inhibiční účinnost na cholinesterázy. V druhém kroku byly stanoveny kinetické parametry. Byly použité elektrochemické senzory za použití 2 podmínek, enzym v roztoku a enzym imobilizovaný. Následně byly vyhodnocené nejúčinnější látky a diskutován vliv imobilizace na kinetické parametry. biosenzory; imobilizace cholinesteras; fenylkarbamaty; inhibice; IC50; kinetické parametry
eng Electrochemical Sensors for the Estimation of the Inhibitory Effect of Phenylcarbamates to Cholinesterase The inhibitory effect of nine phenylcarbamates with various substituents was studied. For this purpose, electrochemical sensors were applied under two different conditions: if an enzyme was present in the solution or if the enzyme was immobilized onto the electrode surface. In both cases, 3-[(ethoxycarbonyl)amino]phenyl (4-chloro-phenyl)carbamate was found as the most effective inhibitor for butyrylcholinesterase. The best inhibitors for acetylcholinesterase were determined as well, depending on the used method. Thus, 3-[(butoxycarbonyl)-amino]phenyl phenylcarbamate with the enzyme present in the solution and 3-[(ethoxycarbonyl)amino]phenyl (3-methylphenyl)carbamate when the enzyme was immobilized onto the electrode surface were evaluated as the most effective inhibitors. Michaelis constants as well as maximum reaction rates were calculated and assessed. biosensors; immobilization of cholinesterases; phenylcarbamates; inhibition; IC50; kinetic parameters

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