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Evaluation of the impact of TiO2 and SiO2 nanofibers on the neuronal cells.
Year: 2019
Type of publication: ostatní - článek ve sborníku
Name of source: Toxicology Letters. vol. 314(S1) : abstracts of the 55th Congress of the European Societies of Toxicology (EUROTOX 2019)
Publisher name: Elsevier
Place: Kidlington
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Evaluation of the impact of TiO2 and SiO2 nanofibers on the neuronal cells. Introduction: Nanomaterials, such as nanoparticles or nanofibers, have a large potential for use in a number of industry sectors. A number of nanomaterials have been used and new types of structures with improved properties are being produced. In particular, inorganic nanofibers produced by electrospinning possess interesting prospects (porous structure, high surface area and breathability, tunable surface reactivity, dimensions). However, their impact on biological systems has not been well characterized yet. Generally, nanomaterials can have potential hepatotoxic and nephrotoxic, but also neurotoxic effects. The consequences of the presence of nanomaterials in the body are not fully understood. Therefore it is necessary to conduct neurotoxicity studies of these materials. Aim: In vitro testing of inorganic electrospun nanofibers, comparing their effect with nanoparticles in neuronal cells. Methods: The effect of inorganic nanofibers (TiO2 and SiO2) was tested comparing with TiO2 P25 nanoparticles in human neuroblastoma SH-SY5Y cell line. The SH-SY5Y cells were seeded into 96-well plates at density of 2.5×104 cells/well and incubated in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 containing tested nanomaterials at concentrations up to 100 µg/ml. The cells were incubated up to 24 hours. The cell viability was tested and evaluated by the WST-1 test spectrophotometrically. Results and Conclusion: We found that none of the tested nanofibers caused a decrease in cell viability after 1 to 6 hours incubation period. After 24 hours of incubation, we found that TiO2 P25 nanoparticles caused a decrease in cell viability below 80% at concentration of 100 µg/ml. Nanofibers, on the other hand, did not induce changes in cell viability. The results on no significant cell damage were also supported by fluorescence microscopic analysis. Acknoledgement: This work was supported by OP RDE project „Strengthening interdisciplinary cooperation in research of nanomaterials an Neurotoxicity; Nanofibers; SH-SY5Y cell line

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