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Biological effects of CdCl2, HgCl2 and ZnCl2 in neuroblastoma cell line

Authors: Tichá Lenka | Čapek Jan | Roušar Tomáš | Handl Jiří

Year: 2023

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Biological effects of CdCl2, HgCl2 and ZnCl2 in neuroblastoma cell line	Heavy metals are elements that vary in chemical properties and biological functions. The group of heavy metals includes essential elements necessary for the proper function of human metabolism, such as copper, selenium or zinc. These heavy metals are important for antioxidant and immune system, or they could be a part of proteins. The group of heavy metals also includes metals that pose a risk of toxicity to the human body, such as arsenic, lead, cadmium, and mercury. These can damage the kidney [1], liver, lung, or brain. Especially in neural cells, cadmium and mercury can induce functional and morphological changes. These heavy metals are widespread in the environment. It is the reason why it is necessary to study their biological effects [2]. Our aim was to characterize the biological effects of ZnCl2, CdCl2 and HgCl2 on dehydrogenase activity and glutathione levels [3] in the SH-SY5Y human neuroblastoma cell line. Cells were exposed to ZnCl2, CdCl2 and HgCl2 for 6 and 24 h. In addition, changes in cell morphology, actin filaments, and cell nuclei were observed after the treatment using phase contrast, Hoechst 33258 and phalloidin-FITC fluorescent probes. We found changes in dehydrogenase activity and glutathione levels after 6 and 24 h of incubation with CdCl2 and HgCl2. After 24 h, we found a transient increase of glutathione levels in cells treated with the lowest tested concentrations 1 µM HgCl2 and 5 µM CdCl2. In conclusion, our results showed that even treatment with 100 µM ZnCl2 does not have any cytotoxic effect in neural cells. On the other hand, CdCl2 and HgCl2 were capable to induce significant toxic effect in neural cells at concentrations under 50 μM, respectively.	CdCl2; HgCl2; ZnCl2; neuroblastoma cell line