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Different biological effects of bacterial and synthetic melanins in neuroblastoma cells

Authors: Handl Jiří | Nývltová Pavlína | Čapek Jan | Roušarová Erika | Knotková Kateřina | Česla Petr | Roušar Tomáš

Year: 2023

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Different biological effects of bacterial and synthetic melanins in neuroblastoma cells	Melanins are heterogeneous dark pigments formed by the polymerization of phenolic and indolic compounds. Their specific structure is rather non-defined. The colour of individual types of melanin can be various, but most of them are coloured black. Melanins have relatively diverse functions and properties [1]. Many commercially available melanins used in scientific studies have been produced synthetically but could be also isolated from natural sources [2]. The aim of our study was to compare the biological effects of two types of melanin, i.e. biotechnologically obtained bacterial melanin (BM) from Bacillus thuringiensis with commercially available synthetic melanin (SM) in vitro. The main aim was to estimate the biological effects of both melanins in the SH-SY5Y neuroblastoma cell line. After melanin treatment (0-200 μg/mL), cell viability, glutathione levels [3] and cell respiration were assessed. The results were supported by images obtained by fluorescent microscopy. Firstly, both tested melanins were structurally characterized in detail and BM was more hydrophilic. After melanin treatment of SH-SY5Y cells, we found that BM induces lower cell impairment compared to SM at similar dose. BM treated cells exhibited similar respiration values as the untreated cells. In addition, more severe toxic effect of SM was found in mitochondria. In general, we conclude that more hydrophilic BM induces lower cell impairment in comparison to SM [4]. Although further elucidation of the toxicity mechanism is needed, it seems that the difference in the level of toxicity induced by melanins can depend on their water solubility. Our findings can be used for neuroscientific studies to estimate the potential use for the study of neuroprotection or neuromodulation. The financial support was received from the Ministry of Education, Youth and Sports of the Czech Republic via projects IT4Neuro(degeneration), Reg. No. CZ.02.1.01/0.0/0.0/18_069/0010054.	Bacterial melanin; Synthetic melanin; Neurotoxicity; Neuroblastoma cells; Mitochondrial impairment