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Establishment of 2D and 3D cell culture models for cadmium neurotoxicity testing

Authors: Tichá Lenka | Čapek Jan | Roušar Tomáš | Handl Jiří

Year: 2024

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Establishment of 2D and 3D cell culture models for cadmium neurotoxicity testing	Cell cultures are widely used as in vitro tools for understanding cell biology, tissue morphology, and toxicity mechanisms. Three-dimensional (3D) cell culturing is a technique for monitoring various cellular mechanisms in toxicology, tissue engineering, and drug development. 3D systems could simulate better in vivo models than two-dimensional (2D) cultures. The 3D system may overcome some limitations of 2D cultures in terms of cell-to-cell communication and cell network formation. The arrangement of cell culture conditions can influence obtained results significantly. Thus, we aimed to compare the biological effect of Cd treatment in the human neuroblastoma SH-SY5Y cell line cultured as a 2D monolayer and in 3D spheres. Cells were exposed to 0-100 µM CdCl2 for up to 48 h. We used the measurement of dehydrogenase activity and glutathione levels to characterize cell impairment. In addition, fluorescence microscopy after staining of actin filaments and cell nuclei was used to detect cell morphology changes. In conclusion, we established a 3D model of SH-SY5Y cell culture. We found changes in dehydrogenase activity and glutathione levels after 24 and 48 h of incubation with CdCl2. After 24 h, 100 µM CdCl2 caused a reduction of dehydrogenase activity in monolayer cultured cells compared to untreated cells. After 24 h, we found a transient increase of glutathione levels in 2D cultured cells treated with 25 µM CdCl2. We also observed changes in 3D cultured cells treated with CdCl2 confirming that 3D cultures of SH-SY5Y cells were less sensitive to CdCl2 effect than 2D monolayer cells.	cell culture; cadmium; neurotoxicity