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Comparison of TiO2 P25 Nanoparticles Effect in 2D and 3D Cultured Neuronal Cells

Authors: Tichá Lenka | Čapek Jan | Báčová Jana | Roušar Tomáš | Handl Jiří

Year: 2024

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Comparison of TiO2 P25 Nanoparticles Effect in 2D and 3D Cultured Neuronal Cells	Titanium dioxide nanoparticles, 25 nm in size of crystallites (TiO2 P25 NPs), are one of the most widely used nanoparticles in industry due to their variability in applications. Because of their small size, they can easily permeate into the human body and cause cell impairment. Our aim was to characterize a biological effect of TiO2 P25 NPs in the human neuroblastoma cell line. Because the arrangement of cell culture conditions can significantly influence the results, we aimed to compare the biological effect of TiO2 P25 NPs treatment in the SH SY5Y cell line cultured as a 2D monolayer and in 3D spheres. Cells were exposed to 0-100 µg·mL 1 TiO2 P25 NPs for up to 48 h. We used the measurement of dehydrogenase activity and glutathione levels to characterize the cell damage. In addition, fluorescence microscopy was used to detect cell morphology changes. In conclusion, we established a 3D model of SH-SY5Y cell culture. We found changes in dehydrogenase activity and glutathione levels after 24 and 48 h of incubation with TiO2 P25 NPs. We also observed changes in 3D cultured cells treated with TiO2 P25 NPs confirming that 3D cultures of SH-SY5Y cells were less sensitive to TiO2 P25 NPs effect than 2D monolayer cells.	TiO2 P25 Nanoparticles; Neuronal Cells; 2D culture; 3D culture