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CHANGES OF ICAM-1 MOLECULE EXPRESSION IN 2D AND 3D MODELS OF SH‑SY5Y CELLS AFTER CISPLATIN TREATMENT

Authors: Čapek Jan | Handl Jiří | Tichá Lenka | Mentlová Denisa | Roušar Tomáš

Year: 2025

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	CHANGES OF ICAM-1 MOLECULE EXPRESSION IN 2D AND 3D MODELS OF SH‑SY5Y CELLS AFTER CISPLATIN TREATMENT	Cell surface and transmembrane glycoproteins are essential for many cellular processes such as migration, adhesion, and immune responses. The intercellular adhesion molecule ICAM-1 (CD54) is a glycoprotein found on the cell surface. Specifically, it is a regulator of cellular processes and plays an important role in the regulation of tissue integrity and homeostasis. The ICAM-1 molecule is expressed very little under physiological conditions [1]. Increased expression of the ICAM-1 molecule is observed during inflammation, where it has a positive effect on leukocyte adhesion to endothelial cells. In addition, expression of ICAM-1 plays an important role in inducing neuro‑inflammation [2,3]. The aim of our study was to compare the changes in expression of ICAM-1 in neuroblastoma SH‑SY5Y cells in 2D and spheroids arrangements after 5 and 8 days of incubation with cisplatin (0.1–10 μg/mL). For visualization of ICAM-1, we used fluorescence microscopy. Quantification of ICAM-1 expression was performed using the Simple Wester method. We detected and evaluated the positive changes in the expression of ICAM-1 in 2D and spheroids of SH‑SY5Y cells after 5 and 8 days of incubation with cisplatin compared to untreated cells. In spheroids of SH‑SY5Y cells, we detected higher expressions of the ICAM-1 molecule compared to the 2D model. The increase in ICAM-1 expression was dependent on the duration of incubation of cells with increasing concentration of cisplatin.	ICAM-1; SH‑SY5Y; CISPLATIN