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Studying Toxicity in Human Mesenchymal Chorionic Stem Cells

Authors: Handl Jiří | Strnadová Viktorie | Čapek Jan | Harvanová Denisa | Matejová Jana | Roušar Tomáš

Year: 2025

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Studying Toxicity in Human Mesenchymal Chorionic Stem Cells	Mesenchymal stem cells (MSCs) represent a population of multipotent cells with significant immunomodulatory and regenerative potential. In our study, MSCs obtained from human extraembryonic placental chorion (CHoMSCs) were used. Chorion is an ethically acceptable and relatively available source of MSCs. The aim of our study was to evaluate the cytotoxic effects of cis-diamminedichloroplatinum (CisPt), cadmium chloride (CdCl2), and tert-butyl hydroperoxide (t-BHP, positive control) in CHoMSCs in vitro. We optimized CHoMSC seeding density and monitored population doubling time from 2nd to 13th passages. The tested substances were incubated with CHoMSC cells for 24 hours. Then, advanced bioanalytical methods were used to characterize the level of cytotoxicity of the tested substances. Cell viability was assessed based on intracellular dehydrogenase activity using the resazurin assay. The specific fluorescent probe monochlorobimane was used to determine the intracellular glutathione level reflecting the degree of oxidative stress. Fluorescence microscopy was used to monitor cytoskeletal morphological changes visualized with phalloidin-FITC and cell nuclei visualized with the Hoechst 33258 probe. The results showed concentration-dependent toxic effects of all tested substances. The most pronounced changes in cell morphology and viability were observed after exposure to 100 µM CdCl₂ and 25 µM CisPt. Lower concentrations of the tested substances had no significant effect on viability or morphology of CHoMSC cells. The results were repeatable between 2nd to 7th passages of CHoMSCs. The results confirmed that CHoMSCs represent a suitable model system for in vitro evaluation of the cytotoxicity of various chemical substances.	Mesenchymal Stem Cells; Placental Chorion; Cytotoxicity; Cadmium Chloride; Intracellular Dehydrogenase