Publikace detail

Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids

Autoři: Lísa Miroslav | Holčapek Michal

Rok: 2014

Druh publikace: ostatní do riv

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids	Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the fast separation of 16 nonpolar and polar lipid classes within 6 minute analysis including the partial separation of some species inside individual classes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification of individual species. The composition of esterified fatty acids and polar head groups are characterized based on their characteristic fragment ions obtained from data independent analysis with high and low collision energy. IM is used as an additional separation dimension enabling the separation of lipid classes together with the separation of individual lipid species differing in acyl chain lengths and the number of double bonds coeluting in SFC. Developed SFC/IM-MS method is applied for the comprehensive analysis of lipid composition in real lipid samples. Obtained data from analyzed samples are processed using Progenesis QI software followed by the statistical analysis. Fast SFC analysis is developed for the separation of nonpolar and polar lipid classes in one analysis followed by IM-MS identification of individual species. Developed SFC/IM-MS approach enables high-throughput nontargeted lipidomic analysis of complex lipid samples. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic).
eng	Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids	Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the fast separation of 16 nonpolar and polar lipid classes within 6 minute analysis including the partial separation of some species inside individual classes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification of individual species. The composition of esterified fatty acids and polar head groups are characterized based on their characteristic fragment ions obtained from data independent analysis with high and low collision energy. IM is used as an additional separation dimension enabling the separation of lipid classes together with the separation of individual lipid species differing in acyl chain lengths and the number of double bonds coeluting in SFC. Developed SFC/IM-MS method is applied for the comprehensive analysis of lipid composition in real lipid samples. Obtained data from analyzed samples are processed using Progenesis QI software followed by the statistical analysis. Fast SFC analysis is developed for the separation of nonpolar and polar lipid classes in one analysis followed by IM-MS identification of individual species. Developed SFC/IM-MS approach enables high-throughput nontargeted lipidomic analysis of complex lipid samples. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic).	Lipidomics; Supercritical fluid chromatography; ion mobility; mass spectrometry; lipids

Vyhledávání

Přihlášení pro studenty

Přihlášení pro zaměstnance

Publikace detail

Katedry

Ústavy a pracoviště

Jak nás najdete?

Služby

Často hledáte