Publikace detail

New Trends in Qualitative Analysis of Protein

Autoři: Bílková Zuzana | Slováková Marcela | Korecká Lucie | Minc Nicolas | Viovy Jean-Louis

Rok: 2005

Druh publikace: ostatní - přednáška nebo poster

Název zdroje: 11th International symposium on separation sciences

Název nakladatele: Univerzita Pardubice

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Jazyk	Název	Abstrakt	Klíčová slova
cze	Nové trendy v kvalitativní analýze proteinů.	Extrakce epitopů - nástroj ke screeningu potencionálních peptidů vhodných pro vakcínu, za použití proteolytického magnetického reaktoru kombinovaného s FT-ICR MS.	trypsin; magnetické mikročástice; Ab (1-40) peptid; epitopová extrakce; imunosorbent; FT-ICR-MS; struktura vakcíny
eng	New Trends in Qualitative Analysis of Protein	Affinity chromatography utilizes of the specific interactions between two molecules for the purification, determination, identification of a target molecule. In practice, a ligand having the affinity for a target molecule is covalently attached to an insoluble support and functions as bait for capturing the target from complex solutions. Ligands that have been used for affinity separations include small organic compounds that are able to dock into binding sites on proteins, inorganic metals that form coordination complexes with certain amino acids in proteins, hydrophobic molecules that can bind nonpolar pockets in biomolecules, proteins with specific binding regions that are able to interact with other proteins, and antibodies, which can be designed to target any biomolecule through their antigen binding sites. A support or matrix is any material to which a biospecific ligand may be covalently attached. Useful affinity supports are those with a high surface area to volume ration, chemical groups that are easily modified for covalent attachment of ligands, minimal nonspecific binding properties, good flow characteristics and mechanical and chemical stability. The concept of using immobilized affinity ligands has extended beyond just chromatographic applications. Affinity ligand are now coupled to latex beads, nanoparticles, macro-beads, membranes, microplates, array surfaces, dipsticks and a host of other devices, that facilitate the capture of specific biomolecules, proteins. The application of affinity targeting includes purification, scavenging (or removal of contaminants), catalysis (or modification of target molecules) and a broad range of analytical uses, the microspheres have also found use in diagnosis, therapy and drug targeting. Using carriers with magnetic property overcomes many of the problems associated with the use of liquid gel slurries in high-throughput and standard laboratory applications. Magnetic particles provide universal system with addi	trypsin; magnetic microspheres; Ab (1-40) peptide; epitope extraction; immunosorbent; FT-ICR-MS; vaccine lead structure

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