Publikace detail

Study of acetaminophen hepatotoxicity – the toxic effect of acetaminophen-glutathione conjugate on mitochondrial respiration.

Rok: 2010

Druh publikace: ostatní - přednáška nebo poster

Název zdroje: Česká a slovenská gastroenterologie a hepatologie

Název nakladatele: Česká lékařská společnost J. E. Purkyně

Místo vydání: Praha

Strana od-do: S27

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Study of acetaminophen hepatotoxicity – the toxic effect of acetaminophen-glutathione conjugate on mitochondrial respiration.	Currently, we reported that APAP-SG is able to inhibit glutathione reductase, an enzyme that accounts for backward reduction of glutathione disulphide. In the present work, we aimed to follow these results so that we estimated an influence of specific acetaminophen metabolite, APAP-SG on mitochondrial respiration and ROS production. We found that APAP-SG conjugate possessed an inhibition effect on mitochondrial respiration in both complex I and II. APAP-SG (5 mM) decreased the rate of respiration to 26 % and 55 % in complex I and II, respectively. An inhibition effect on complex I activity was found even in 0.2 mM APAP-SG (to 57 % of control signal); however, the complex II activity was not decreased at 0.2 mM APAP-SG. To estimate the level of intramitochondrial ROS production we used DCFDA probe. We proved that ROS formation was enhanced in the presence of APAP-SG and succinate as a substrate: to 900 % and 350 % of control signal, in 5 mM and 1 mM APAP-SG, respectively.	acetaminophen; hepatotoxicity; mitochondrial; respiration; glutathione

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