Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Analysis of Free Amino Acids in Honey by Capillary Zone Electrophoresis

Autoři: Fischer Jan | Staněk Václav | Weiserová Hana

Rok: 2010

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Analysis of Free Amino Acids in Honey by Capillary Zone Electrophoresis	CZE with UV detection (254 nm) was used for analysis of free amino acids occurring in different honey samples. Standard solutions of 20 amino acids and sample solutions were precapillary derivatized by PITC using modified method described in and then analyzed using non-coated fused silica capillaries and phosphate buffers as background electrolytes. The best separation selectivity of PITC derivatives was achieved in 30 mmol/L phosphate buffer (pH = 7.4) containing 4 mmol/L β-cyclodextrin. Limit of detection and limit of quantitation was calculated from the calibration measurements. Amino acid content was evaluated in commercial honey samples and samples acquired from private bee-keepers. Finally, results obtained by PITC-derivatization method were compared with direct CZE sample analysis (no derivatization step, detection UV at 190 nm).