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Optimization of Selectivity, Peak Capacity and Separation Time in Two-Dimensional HPLC by Matching Separation Systems and Adjusting Gradient Conditions in the Two Dimensions

Autoři: Jandera Pavel | Hájek Tomáš | Česla Petr

Rok: 2011

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Optimization of Selectivity, Peak Capacity and Separation Time in Two-Dimensional HPLC by Matching Separation Systems and Adjusting Gradient Conditions in the Two Dimensions	Gradient elution provides significantly higher peak capacity in comparison to isocratic elution mode and enables separation of sample components over a broader retention range with improved resolution. This advantage is especially important in comprehensive two-dimensional LCxLC employing orthogonal systems with great differences in selectivity in the first and in the second dimension. The gradient time in the first dimension is adjusted according to the total analysis time. However, the second-dimension analysis time is strictly limited by the fraction modulation cycle and usually should be as short as 1 min or even less. Hence, the selection of gradient conditions in the second dimension is crucial for the success of the twodimensional separation. The factors affecting the selection of the second dimension gradient program are discussed. The gradient time and range should be selected to achieve maximum resolution and regular coverage of the retention space throughout the whole 2D separation.	Optimization; Selectivity; Peak Capacity; Two-Dimensional Liquid Chromatography

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