Publikace detail

Optimization of Second Dimension Gradient Separation Conditions in Two-dimensional LCxLC and LC-MEKC

Autoři: Česla Petr | Hájek Tomáš | Fischer Jan | Jandera Pavel

Rok: 2011

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Optimization of Second Dimension Gradient Separation Conditions in Two-dimensional LCxLC and LC-MEKC	In 2D LCxLC employing RP separations in both dimensions, gradient elution improve the peak capacity with respect to isocratic conditions, but 2nd dimension gradients are limited to time equal or less to the fraction collection time. Different types of 2nd dimension gradients were applied in separations of antioxidants on a PEG column in 1st dimension and porous shell fused-core C18 columns in 2nd dimension. In 2D separations combining LC in 1st dimension with MEKC in 2nd dimension, programmed separation conditions can be used to compensate the shifts in migration times in 2nd dimension MEKC separation caused by the changing composition of mobile phase transferred from LC. The shifts in migration times increase with increasing length of the injected sample zone, i.e. during the on-line preconcentration step and can be minimized by changing composition of pseudostationary phase in BGE.	Optimization; Second Dimension Gradient; 2D LCxLC; 2D LC-MEKC

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