Publikace detail

On-line two-dimensional liquid chromatography-capillary electrophoresis separation of phenolic compounds

Autoři: Česla Petr | Dinisová Petra | Fischer Jan | Jandera Pavel

Rok: 2011

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	On-line two-dimensional liquid chromatography-capillary electrophoresis separation of phenolic compounds	In this work, new approach to on-line 2D separation method combining capillary liquid chromatography with capillary electrophoresis is presented. Capillary columns with fused core C8 and C18 silica particles were applied in the first separation dimension. In the second dimension, the short capillaries with extended light path cells were applied for fast separation of compounds in fractions transferredfrom LC separation. The transfer of the fractions between the two dimensions is based on the application of electronically controlled switching valve. The separation conditions in both LC and CE dimensions were optimized using a set of representative standards of phenolic compounds. Further, optimized programmed separation conditions in both dimensions were used to improve the sample resolution and peak capacity in comparison to the isocratic system. The on-line 2D method under optimal conditions was applied on the separation of phenolic compounds in environmental and food samples.	on-line two-dimensional liquid chromatography-micellar electrokinetic chromatography; 2D separations; flavones; phenolic acids

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