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Characterization of praziquantel and its metabolites by ultra-performance liguid chromatography coupled with electrospray tandem mass spectrometry

Autoři: Jedličková Veronika | Jirásko Robert | Vokřál Ivan | Skálová Lenka | Holčapek Michal

Rok: 2011

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Characterization of praziquantel and its metabolites by ultra-performance liguid chromatography coupled with electrospray tandem mass spectrometry	In our work, PZQ was incubated with rat hepatocytes and microsomes. Subsequently, the solid-phase extraction of microsomal and hepatocyte incubation mixtures was performed. Moreover, rat urine samples were obtained from in vivo experiments after PZQ oral administration. The comparison of incubation samples with placebo experiments simplified the identification of metabolites. Studied samples were analyzed using optimized UHPLC coupled with electrospray ionization tandem mass spectrometry method that provided information about PZQ microsomal and hepatic biotransformation pathways. Structures of particular phase I and II metabolites were suggested based on the high mass accuracy measurements in full scan and tandem mass spectrometry modes using QqTOF analyzer. The additional measurements of MSn using ion trap analyzer were performed for the verification of fragmentation paths.	praziquantel;drug metabolites;UHPLC/MS/MS

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