Univerzita PardubiceFakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

HPLC/MS Lipidomic Characterization of Human Plasma

Autoři: Holčapek Michal | Cífková Eva | Lísa Miroslav | Netušilová Kateřina

Rok: 2011

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	HPLC/MS Lipidomic Characterization of Human Plasma	It is possible to obtain the important information about the composition of lipids in healthy or ill individuals by the HPLC/MS analysis of the total lipid extract. For this purpose, we develop a new method for the characterization of the lipid composition in the biological tissues. This HPLC/MS/MS is developed together with the complementary information from conventional gas chromatography-flame ionization detection profiling of fatty acids after the tranesterification of lipid extracts. The total extract of plasma was fractioned using hydrophilic interaction liquid chromatography (HILIC) into individual lipid classes in the first dimension. The use of sub-2 um particles with ultrahigh-performance liquid chromatography (UHPLC) reduces the analysis time.	Lipidomics;plasma;HPLC/MS;GC/FID;UHPLC/MS