Univerzita PardubiceFakulta chemicko- technologická

Univerzita Pardubice

Zaměstnanci
|
Studenti
English

Publikace detail

Determination of α-tocopherol and retinol in human seminal plasma

Autoři: Drábková Petra | Hampl Radek | Kanďár Roman

Rok: 2012

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Determination of α-tocopherol and retinol in human seminal plasma	α-Tocopherol and retinol are lipid-soluble vitamins. Both play important role in reproduction. Retinol is required for male reproduction. Its metabolite, retinoic acid, is need for spermatogonial differentiation and the entrance into meiosis. α-Tocopherol is an antioxidant which is able to scavange peroxyl radicals. This way it inhibits chain reaction leading to the production of lipid peroxides which can have a deleterious effect on the biological membrane of sperm and its function. The aim of this study was to develop a method for the simultaneous determination of α-tocopherol and retinol in human seminal plasma using an HPLC. Semen samples were obtained from male partners of couples presenting for a fertility evaluation in Sanus, In Vitro Fertilization Clinic, Pardubice. After liquefaction, semen samples were divided in two portions. The first portion was used to evaluate sperm characteristics according to World Health Organization guidelines (2010). The second portion of semen sample was centrifuged and supernatant (seminal plasma) was separated and stored at – 80 °C until analysis. For the determination of α-tocopherol and retinol in seminal plasma, a reversed-phase HPLC with isocratic elution was used. Mobile phase was 93.5 % ethanol in deionized water. Samples of seminal plasma were deproteinized with ethanol. Fat-soluble vitamins were extracted twice with n-hexane. The hexane extracts were evaporated under nitrogen at 60 °C and residue was dissolved in ethanol. α-Tocopheryl acetate and retinyl acetate were used as internal standards. Analytical performance of this method is satisfactory for both α-tocopherol and retinol. Acknowledgements: The Ministry of Education (Grant SGFChT 07/2012) supported this work.	α-tocopherol; retinol; human seminal plasma; HPLC