Univerzita PardubiceFakulta chemicko- technologická

Univerzita Pardubice

Zaměstnanci
|
Studenti
English

Publikace detail

MAGNETIC BEADS-BASED MICROFLUIDIC SYSTEMS: THE PAST AND THE FUTURE

Autoři: Bílková Zuzana | Svobodová Zuzana

Rok: 2012

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	MAGNETIC BEADS-BASED MICROFLUIDIC SYSTEMS: THE PAST AND THE FUTURE	In recent years, nanotechnologies have greatly impacted biotechnological research and analytical technologies for genomics, proteomics, clinical diagnostics, cell-based assays, disease screening and drug discovery. Many of such applications have potential to bring the analysis closer to patient to evaluate immediately level of certain biomarkers 1. Special devices enabling such analysis are called micro-total analysis systems (μ-TAS), lab-on-chips (LOC), point of care devices (POCT) or biomicrodevices 2. Development of such microfluidic system requires complex knowledge of physics, microfabrication techniques and surface chemistry, moreover methods for analysis of biological material and medical background. Interestingly, in last two decades, microfluidic devices based on affinity magnetic separations using magnetic micro/nanoparticles were developed and successfully applied in all aforementioned fields of bioanalysis 3. The magnetic particles have most frequently spherical monodisperse shape and are made magnetisable and/or superparamagnetic, meaning they are only magnetic in a magnetic field. Employing magnetic beads in microfluidic devices brings broad range of advantage. Beads-based immunoassays (e.g. particle capture ELISA) 4, bead-based microfluidic enzyme catalysis 5 or self-assembled beads-based channels for cell sorting 6, bead-based DNA in-microchip analysis 7, multiplex assays (e.g. Luminex), digital microfluidic platform (DMF) 8, droplet microfluidics 9 and biobarcoded magnetic beads assays 10 are selected examples documenting the strength of this arrangement.	MAGNETIC; BEADS-BASED; MICROFLUIDIC; IMMUNOASSAYS