Přihlášení pro studenty

Přihlášení pro zaměstnance

Publikace detail

Characterization of Sambucus nigra L. Infusions Using Micellar Electrokinetic Capillary Chromatography and Liquid Chromatography-Tandem Mass Spectrometry
Autoři: Váňová Jana | Dinisová Petra | Česlová Lenka | Fischer Jan | Česla Petr
Rok: 2014
Druh publikace: ostatní - přednáška nebo poster
Strana od-do: nestránkováno
Tituly:
Jazyk Název Abstrakt Klíčová slova
eng Characterization of Sambucus nigra L. Infusions Using Micellar Electrokinetic Capillary Chromatography and Liquid Chromatography-Tandem Mass Spectrometry Inflorescence of Sambucus nigra L. is used in the form of water infusions to treat the symptoms of influenza, colds and sinusitis due to their diaphoretic properties [1]. The biological effects can be attributed to the presence of plant metabolites, which are mainly (poly)phenolic compounds. In the present work, we have developed and compared two methods for the determination of the content of (poly)phenols in Sambucus nigra L. infusions. The liquid chromatographic method based on reversed-phase separations using octadecyl silica gel column packed with fused-core particles was employed in combination with the electrospray ionization tandem mass spectrometric detection n the negative-ion mode. The individual compounds were identified based on the multiple reactions monitoring of characteristic transitions in the mass spectra. The second micellar electrokinetic capillary chromatography method was employed using non-coated fused silica capillary and sodium decyl sulfate micellar agent, which offers increased selectivity and speed of the analysis for the separation of (poly)phenols in comparison to the most commonly used sodium dodecyl sulfate [2]. The parameters of the quantitative analysis and the results were compared using analysis of variance and the effect of the sample preparation on the content of (poly)phenols in water infusion was evaluated. The results for both methods were correlated and the differences are discussed with the aim to provide a tool for the comprehensive characterization of the herbal infusion samples. Sambucus nigra L.; Micellar Electrokinetic Capillary Chromatography; Liquid Chromatography-Tandem Mass Spectrometry

Katedry

Ústavy a pracoviště

Jak nás najdete?

zobrazit na mapy.cz

Služby

Často hledáte

© 2023 Univerzita Pardubice, Studentská 95, 532 10 Pardubice 2
Telefon: 466 036 111, 466 036 112, 466 036 113
Správce webu, RSS
ID datové schránky: f5vj9hu
Prohlášení o přístupnosti
CC BY-NC-ND 4.0 CZCreative CommonsAttributionNonCommercialNoDerivatives