Publikace detail

Application of immobilized trypsin reactors as proteomics tool. Techniques and procedures for disease diagnostics

Autoři: Slováková Marcela

Rok: 2014

Druh publikace: kapitola v odborné knize

Název zdroje: Techniques and procedures for disease diagnostics

Název nakladatele: LAP Lambert Academic Publishing AG & Co. KG

Místo vydání: Saarbrücken

Strana od-do: 107-114

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Aplikace imobilizovaného trypsinu v proteomice	Trypsin je jedním z nejčastěji používaných imobilizovaných proteináz v proteomice. Použití trypsinu imobilizovaného na celulózová nanovlákna a magnetické nanočástice z SiO2 k proteolýze modelových proteinů (alfa-kaseinu a hovězího albuminu).	imobilizovaný trypsin;proteomika
eng	Application of immobilized trypsin reactors as proteomics tool. Techniques and procedures for disease diagnostics	In proteomics study, trypsin is obviously the most often used immobilized proteinase for the preparation of peptide maps. We immobilized trypsin on cellulose nanofibers and on magnetic silica nanoparticles. Both enzyme reactors were applied for proteolytic digestion of model proteins (α-casein and bovine albumin). Analysis showed that immobilized trypsin is well usable and comfortable. There were good consensuses within protein recognition in comparison with soluble trypsin. Sequence coverage within magnetic silica nanoparticles functionalized by trypsin was higher than 47.6%. Sequence coverage for trypsin-nanofibers could not be determined due to high number of non-specific fragments.	immobilized; trypsin; proteomics

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