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ANALYSIS OF FATTY ACIDS COMPOSITION IN HDL FRACTION OF EDTA PLASMA OF TYPE 2 DIABETIC PATIENTS

Autoři: Laštovička Petr | Líbalová Martina | Čermák Tomáš | Mužáková Vladimíra | Čegan Alexander

Rok: 2014

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	ANALYSIS OF FATTY ACIDS COMPOSITION IN HDL FRACTION OF EDTA PLASMA OF TYPE 2 DIABETIC PATIENTS	Diabetes is a long-term problem in developed countries. Numbers of patients with this disease have not risen only because of poor lifestyle (which remains a major problem), but also because of the development of medicine and earlier diagnostics. Many studies have focused on LDL. However, on the cholesterol deposition and the resulting complications in diabetics participates disruption of lipid metabolism and fatty acid composition of HDL. The ability to reverse cholesterol-transport by HDL decreases with increasing number of VLCFA and SFA in these particles. 21 anonymized plasma samples randomly selected type 2 diabetic patients and 10 healthy blood donors plasma samples as a control group were processed. The group of diabetic patients were categorized into two groups according to the level of glycated haemoglobin (HbA1c). This indicator appears to be most reliable because it reflects compesation of diabetes and highlights the development of blood glucose in the last 4 to 6 weeks prior to collection. HDL was obtained by ultracentrifugation of EDTA plasma samples. HDL lipids were divided into five fractions by TLC and analysed by GC. The data were processed in STATISTICA 12. Differences in variables between the groups were evaluated using t-test. P value less than 0.05 was considered statistically significant. For investigating of correlations were used pearsons correlations. Negative correlation of elongase index with HbA1c in diabetics in phospholipids (PL) (r = -0,47; p = 0,032) and free fatty acids (FFA) (r = -0,48; p = 0,029) was found. Negative relationship with HbA1c was found in case of arachidic acid (r = -0,47; p = 0,029) in PL, there occurred lower concentration of arachidic acid in decompensated diabetics compared to healthy donors (0,28% vs. 0,20%; p = 0,019). In cholesterol ester (CE) fractionwe found lower level of nervonic acid in compensated diabetics in comparison to healthy donors (0,58% vs. 0,30%; p = 0,023).	diabetes mellitus, HDL, elongase, gas chromatography, thin layer chromatography, stearic acid

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